Signature of John O Yoho

The above signature is a copy of a copy so the quality is not good.

The John Yoho of this signature clearly has an "O" initial for a middle name.

The name is followed by what appear to be initials "J o Y"

Under the name is another signature that may be "A Machir" for Alexander Machir

Preceeding John's name is the word "His" surrounded by a scrolled loop

Source:

Deed Book 15, Page 233
County Clerk, Fredrick County
Winchester, VA.